Session Title: Neurodevelopment and Neurodegeneration
DEVELOPMENT NEUROTOXICOLOGICAL RISK ASSESSMENT OF THE MANGANESE (DNTRAM): ROLE OF CHEMICAL SPECIATION AND CELL TYPE
R. Bonne Hernandez1, M. Farina2, B. Pannia Espósito1, C. Suñol2
The trace Mn is essential for normal development of all living systems, but may be toxic in some instances, especially neurotoxic. In this way, it have associated with several neurodegenerative diseases such as Parkinson's, Manganism, Alzheimer, Autism and other, but the role of the Mn in its remain unclear. For this reason, our study was to investigate the toxic effects of the oxidation state and chemical form of the Mn in cultured of neocortical (CTX) and cerebellar granular (CGC) neurons, which were exposed to 0-1000 µM, for 120 h, with or without ascorbic acid (AA) and d,l-lactate (L) as neuroprotectors. This report suggests that, energy impairment could be more significant than reductive-oxidative stress in manganese development neurotoxicity (Mn-DNT). Besides, the brain cell type and its origin could be as important as the manganese speciation to Mn-DNT. In this respect, Mn was more toxic and teratogenic to CGC (EC50 = 25 - 40 µM) than CTX (EC50 = 136 - 550 µM) as well as undifferentiated cells were more sensible than differentiated cells, where Mn as divalent specie, presumably an aqua-hydroxi complexe, was more toxic than other such as (Mn(II)Citrate, Mn(III)Citrate and Mn(Pyrophosphate). These collective results can provide several information's to manganese development neurotoxicity risk assessment, (DNTRAM).