Session Title: Neurodevelopment and Neurodegeneration
SCREENING OF POTENTIAL NEURONAL CELL DEATH MODULATORS IN DYRK1A-OVEREXPRESSING HIPPOCAMPAL CELLS
Y. Oh, J. Park, K.C. Chung
Most patients with Down syndrome (DS) show early-onset Alzheimer´s disease (AD). Some recent studies on dual-specificity tyrosine-phosphorylation regulated kinase (Dyrk1A), one of Down syndrome critical region (DSCR) gene products, showed Dyrk1A-mediated phosphorylation of APP and hyperphosphorylation of tau that is linked to AD pathology. In the present we examined the differential protein and mRNA profiles that responding to serum deprivation stimulus of embryonic hippocampal neural progenitor (H19-7) cells that overexpress Dyrk1A in a stable manner. Modified H19-7 cells appeared to be less viable than control cells under the condition of serum deprivation. The results of proteomic and microarray approaches gave us several landmark genes which are generally induced by apoptotic cell death signaling pathway and novel cell death modulators which is closely linked with the neurobiological abnormalities of DS and AD. The detailed data will be presented at the conference.