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<title>Alzheimer's Conference / Parkinson's Conference: ADPD 2009 - Prague, March 11-15 - Poster Presentations</title>
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    <td class="content"><h1>Poster Presentations</h1>
    <P><b>Session Title:</b> Neurodevelopment and Neurodegeneration<br><b>Presentation Date:</b> Friday, March 14 – Saturday, March 15, 2009</P><h2 align='left'><b>CYCLIN-DEPENDENT KINASE-5 (CDK5) PHOSPHORYLATES CDH1 LEADING TO CYCLIN B1 ACCUMULATION DURING EXCITOTOXIC NEURODEGENERATION</b></h2>
<p align='left'>C. Maestre<sup>1</sup>, J.C. Gomez-Sanchez<sup>2</sup>, J.P. Bolaños<sup>3</sup>, <b>A. Almeida</b><sup>1</sup><br>
<em><sup>1</sup>Hospital Universitario de Salamanca, Unidad de Investigacion, Salamanca, Spain, <sup>2</sup>Hospital Universitario de Salamanca, Servicio de Neurologia, Salamanca, Spain, <sup>3</sup>Universidad de Salamanca, Bioquimica y Biologia Molecular, Salamanca, Spain</em></p><br>
<p align='justify'>Anaphase Promoting Complex (APC/C), an E3 ubiquitin ligase that destabilizes cell cycle proteins, is activated by Cdh1 in post-mitotic neurons, where it regulates axonal growth, synaptic plasticity and survival. The APC/C-Cdh1 substrate, cyclin B1, has been found to accumulate in degenerating brain areas in Alzheimer's disease and stroke. This highlights the importance of elucidating cyclin B1 regulation by APC/C-Cdh1 in neurons under stress conditions relevant to neurological disease. Here, we report that stimulation of N-methyl-D-aspartate receptors (NMDAR) that occurs in neurodegenerative diseases promoted the accumulation of cyclin B1 in the nuclei of cortical neurons; this led the neurons to undergo apoptotic death. Moreover, we found that the Ser-40, Thr-121 and Ser-163 triple phosphorylation of Cdh1 by the cyclin-dependent kinase-5 (Cdk5)/p25 complex was necessary and sufficient for cyclin B1 stabilization and apoptotic death after NMDAR stimulation. These results reveal Cdh1 as a novel Cdk5 substrate that mediates cyclin B1 neuronal accumulation during excitotoxic neurodegeneration. (Funded by FIS, RENEVAS and MCINN, Spain).<br></p>
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