Session Title: Neurodevelopment and Neurodegeneration
IN VITRO ACETYLCHOLINESTERASE INHIBITORY ACTIVITY OF MAHANIMBINE IN MURRAYA KOENIGII
N. Satheesh Kumar, P.K. Mukherjee
Alzheimer´s disease (AD) is a neurodegenerative disease characterized by progressive cognitive deterioration together with declining activities of daily living and neuropsychiatric symptoms or behavioral changes. It is the most common cause of dementia. The leaves of Murraya koenigii Spreng commonly called as Curry leaves, is traditionally used in the indigenous system of preventive medicine as dietary ingredient in different parts of India and other Asian countries. In this study we investigated Acetylcholinesterase inhibitory activity of mahanimbine (1) a carbazole alkaloid isolated from Murraya koenigii were tested in vitro AChE inhibitory activity was studied based on the Ellman's method using 96-well microplates. The IC50 values were obtained for the methanolic extract, petroleum ether extract, chloroform extract and mahanimbine were 149.96± 9.10µg/ml, 69.91± 3.70µg/ml,119.74±1.14 µg/ml and 83.11± 8.15µM respectively. Galantamine was used as standard inhibitor with an IC50 value of 1.27 ± 0.21µM. These results states that the petroleum ether extract and its chemical constituent's mahanimbine (1) showed better inhibitory effect on AChE.