The Retinal Thickness Analyzer (RTA, Talia Technologies Ltd, Mevaseret Zion, Israel), is a novel noninvasive instrument developed for multiple optical cross-sectional retinal visualisation that provides quantitative measurements of retinal thickness. The RTA operates on the principle of laser biomicroscopy covering a zone of about 20° X 20° around the fovea. Twelve patients (6 males and 6 females) affected with bilateral primary open angle glaucoma controlled with hypotensive medication and ten healthy subjects (6 males and 4 females) were enrolled in this clinical study. and one eye per person, was randomly selected. The posterior pole average thickness (PPA), posterior pole pattern deviation (PPPD) and posterior pole corrected pattern deviation (PPCPD)indices were used in the present study to assess the intrasession and intersession reproducibility.Reproducibility was calculated using the coefficient of variation(CV(. The intravisit reproducibility was 3.4% for PPA, 5.3% for PPPD and 4.9% for PPCPD in glaucomatous patients and 3.1% for PPA, 4.6% for PPPD and 4.3% for PPCPD in normal subjects. the intervisit reproducibility of RTA measurements: the CV of PPA ranged from 5.6% in glaucomatous patients to 4.8% in healthy subjects. The results of the present study indicate that RTA is highly reproducible method for measuring retinal thickness.