Structural losses of nerve fiber layer and ganglion cell tissue in glaucoma are evaluated in the papillary and peripapillary regions by most glaucoma imaging instruments. However, these regions have large variability in normal eyes. Furthermore, most retinal ganglion cells, notably, those responsible for central vision, are located in the macular region. We explored the use of retinal thickness (RT) measurements in the 6x6 mm area centered on the fovea to assess glaucomatous tissue loss. The Retinal Thickness Analyzer (Talia Technologies, Israel) was used to obtain RT maps in 29 normal eyes (29 persons, mean age 44 y) and 21 eyes (15 persons, mean age 54 y) with Glaucoma Hemifield Test “outside normal limits”. Volume of significant RT loss volume was calculated for superior and inferior hemifields and compared with extent of visual field loss in the same region (defined by probability plot of the corrected pattern deviation). RT volume loss >=12,000 microns per pixel-squared in the inferior hemisphere correctly identified 87% of eyes with 4 or more abnormal visual field points and false-positively occurred in only 10% of normal eyes. Corresponding results in the superior hemisphere were 88% and 17%, respectively. Conclusion: RT measurements in the macular region may help to identify persons with glaucomatous ganglion cell loss and may be a promising way to monitor changes over time in glaucomatous eyes. CR: P (RZ)