Poster Presentations

Thursday, August 21, 2008

Novel Technologies and Methods: Functional Community Analysis

0213
METAPROTEOMIC ANALYSIS OF THE RESPONSES OFANAEROBIC METHANOGENIC CONSORTIA TO LOW TEMPERATURE
F. Abram, J. O'Reilly, T. Mahony, G. Collins, V. O'Flaherty
National University of Ireland Galway, Galway, Ireland

0214
DELVING INTO DIAZOTROPHS WITH ¹⁵N₂-RNA STABLE ISOTOPE PROBING
S. Addison¹, I. McDonald², G. Lloyd-Jones^1
1Scion, Eco-Smart Technologies, Rotorua, New Zealand, ²University of Waikato, Department of Biological Sciences, Hamilton, New Zealand

0215
DEVELOPMENT OF UNCULTIVATED MICROBIAL GENOME-PROBING MICROARRAYS USING DIGITAL MULTIPLE DISPLACEMENT AMPLIFICATION OF SINGLE CELLS
J.-W. Bae¹, H.-W. Chang¹, K.-H. Kim¹, Y.-D. Nam¹, S.W. Roh¹, J.-H. Yoon¹, C.O. Jeon², H.-M. Oh^1
1KRIBB (Korea Research Institute of Bioscience & Biotechnology), Daejeon, Korea, Republic of, ²Chung-Ang University, Seoul, Korea, Republic of

0216
QUANTITATIVE PROTEOMIC ANALYSIS OF MULTI-SPECIES BIOFILM COMMUNITIES
C. Belnap¹, C. Pan², N. VerBerkmoes², M. Power¹, N. Samatova², R. Hettich², J. Banfield^1
1University of California, Berkeley, United States of America, ²Oak Ridge National Laboratory, Oak Ridge, United States of America

0217
EFFECTS OF AN OLIGOCHEATE BLOOM ON THE BACTERIAL COMPOSITION OF A MUNICIPAL ACTIVATED SLUDGE REACTOR
K. Biswas¹, S. Kumarasingham², S. Turner^1
1University of Auckland, School of Biological Sciences, Auckland, New Zealand, ²WaterCare Services Ltd, Auckland, New Zealand

0218
CASE STUDY IN GEOBIOLOGICAL SYSTEMS: IN SILICO ANALYSIS OF MATERIAL AND ENERGY FLUX IN A HOT SPRING MICROBIAL MAT COMMUNITY
J. Aston¹, K. Brileya¹, R. Carlson², M. Fields¹, R. Gerlach¹, W. Inskeep¹, Z. Jay¹, C. Klatt¹, N. Mallette¹, S. McGlynn¹, S. Montross
¹Montana State University, Thermal Biology Institute, Bozeman, United States of America, ²Montana State University, Chemical and Biological Engineering, Bozeman, United States of America

0219
THE DESIGN AND DEVELOPMENT OF AN OLIGONUCLEOTIDE MICROARRAY TO ASSESS MICROBIALLY MEDIATED SOIL PROCESSES
R. Chapman¹, T. Webster¹, H. Hayden¹, D. Crowley², P. Mele^3
1Department of Primary Industries, Biosciences Research Division, Melbourne, Australia, ²University of California, Riverside, Department of Environmental Science, Riverside, Australia, ³Department of Primary Industries, Biosciences Research Division, Rutherglen, Australia

0220
NEW MICROBIAL MARKERS OF DRINKING WATER CONTAMINATION
E. Chi Fru¹, P. Hunter², A. Grant^1
1University of East Anglia, School of Environmental Sciences, Norwich, United Kingdom, ²University of East Anglia, School of Medicine, Health Policy and Practice, Norwich, United Kingdom

0221
DIVERSITY OF NIFH GENE POOLS IN THE RHIZOSPHERE OF SORGHUM (SORGHUM BICOLOR) TREATED WITH CONTRASTING LEVELS OF NITROGEN FERTILIZER
M.R.R. Coelho¹, M. De Vos², N.P. Carneiro³, I.E. Marriel³, S.N. Jenkins⁴, C.V. Lanyon⁴, A.G. O'Donnell⁴, L. Seldin^1
1Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Prof. Paulo de Góes, Rio de Janeiro, Brazil, ²Wageningen Universiteit, Molecular Ecology Group, Laboratory of Microbiology, Wageningen, Netherlands, ³EMBRAPA/CNPMS - Empresa Brasileira de Pesquisa Agropecuaria, Centro Nacional de Pesquisas de Milho e Sorgo, Sete Lagoas, Brazil, ⁴Newcastle University, School of Biology and Psychology, IRES, Newcastle Upon Tyne, United Kingdom

0222
QUANTITATIVE REAL - TIME PCR ANALYSES OF SULFATE REDUCING BACTERIA IN SWINE MANURE AND THE INHIBITORY EFFECTS OF CONDENSED TANNINS
C. Spence, T. Whitehead, M. Cotta
USDA-ARS, Fermentation Biotechnology Research, Peoria, United States of America

0223
HOW MICROBIAL COMMUNITY STRUCTURE AND FUNCTION RELATE TO EACH OTHER WHEN CHLORINATED COMPOUNDS ARE INVOLVED
M.M. David, M. Mesle, C. Malandain, S. Cecillion, I. Navarro, T.M. Vogel
Environmental Microbial Genomics, Laboratoire AMPERE, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, Ecully, France

0224
GENOMICS OF NITRIFYING COMMUNITIES
M. Di Lorenzo¹, Z. He², J. Zhou², H.J. Laanbroek^1
1Netherlands Institute of Ecology NIOO-KNAW, Microbial Wetland Ecology, Nieuwersluis, Netherlands, ²Institute for Environmental Genomics, Norman, United States of America

0225
FUNCTIONAL REDUNDANCY IN THE MICROBIAL COMMUNITY FROM A FULL SCALE WASTEWATER NITROGEN REMOVAL REACTOR
C. Etchebehere¹, W.J. Sul², Y. Deng³, L. Wu³, J. Zhou³, J. Tiedje^2
1UDELAR-Uruguay, Microbiology, Montevideo, Uruguay, ²Michigan State University, Center for Microbial Ecology, East-Lansing, United States of America, ³Institute for Environmental Genomics, University of Oklahoma, Oklahoma, United States of America

0226
ANNUAL VARIATION IN THE DIVERSITY OF THE AIRBORNE BACTERIAL COMMUNITY AS MEASURED BY CULTURE DEPENDENT AND INDEPENDENT TECHNIQUES
C. Fahlgren, U.-L. Zweifel, A. Hagstrom
Kalmar University, Kalmar, Sweden

0227
SEQUENCING COMPLEX MARINE MICROBIAL METATRANSCRIPTOMES WITH PYROSEQUENCING TECHNOLOGY
J. Gilbert¹, R. Edwards², W. Li³, P. Gilna³, D. Field⁴, I. Joint^1
1Plymouth Marine Laboratory, Plymouth, United Kingdom, ²San Diego State University, Department of Computer Science, San Diego, United States of America, ³California Institute for Telecommunications and Information Technology, San Diego, United States of America, ⁴NERC Centre for Ecology and Hydrology, NERC Environmental Bioinformatics Institute, Oxford, United Kingdom

0228
RNA CLEAVAGE-BASED FINGERPRINTING
M.-L. Gutierrez-Zamora, O. Zemb, M. Manefield, S. Kjelleberg
University of New South Wales, Centre for Marine BioInnovation, Sydney, Australia

0229
WHO IS ACTIVELY DENITRIFYING IN ACTIVATED SLUDGE?
A.A. Hansen, J.L. Nielsen
Aalborg University, Section of Biotechnology, Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Denmark, Aalborg, Denmark

0230
CARBON SOURCE UTILIZATION PATTERNS TO DETECT CHANGES IN MICROBIAL COMMUNITIES OF A CRUDE OIL CONTAMINATED SOIL
A. Hernandez, M. Arias
PDVSA INTEVEP, Ambiente e Higiene Ocupacional, Los Teques, Venezuela

0231
ANTARCTIC METAGENOMICS
J. Hoffman¹, T. Thomas², F. Lauro³, M. Brown³, C. Andrews-Pfannkock¹, K. Nealson⁴, J.C. Venter¹, R. Cavicchioli^3
1J. Craig Venter Institute, Environmental Genomics, San Diego, United States of America, ²School of Biotechnological and Biomolecular Sciences, Centre for Marine Bio-Innovation, The University of New South Wales, Sydney, Australia, ³School of Biotechnological and Biomolecular Sciences, The University of New South Wales, Sydney, Australia, ⁴J. Craig Venter Institute, Environmental Genomics, San Diego, Department of Earth Sciences, University of Southern California, Los Angles, United States of America

0232
HOW MANY TARGET MOLECULES ARE NEEDED FOR FISH AND CARD-FISH?
T. Hoshino¹, L.S. Yilmaz², D.R. Noguera², H. Daims¹, M. Wagner^1
1University of Vienna, Department of Microbial Ecology, Vienna, Austria, ²University of Wisconsin, Dept. Civil and Environmental Engineering, Madison, United States of America

0233
BEAD ASSAY BASED SIMULTANEOUS DETECTION OF PATHOGENIC BACTERIA IN AQUATIC ENVIRONMENT
T. Ichijo¹, N. Yamaguchi¹, K. Tani², M. Nasu^1
1Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan, ²School of Pharmacy, Osaka Ohtani University, Tondabayashi, Osaka, Japan

0234
EXPLORING ENVIRONMENTAL BIPHENYL DIOXYGENASE GENES BY CLONE LIBRARIES AND PYROSEQUENCING
S. Iwai, B. Chai, W.J. Sul, J.R. Cole, S.A. Hashsham, J.M. Tiedje
Michigan State University, Center for Microbial Ecology, East Lansing, United States of America

0235
DIVERSITY OF FILAMENTOUS BACTERIA IN DUBAI SEWAGE TREATMENT PLANT
S.M. Faheeem, M.A. Khan, A. Arora
Manipal University Dubai Campus, Biotechnology, Dubai, United Arab Emirates

0236
PROKARYOTIC DIVERSITY IN THE PLANT PHYLLOSPHERE AND ANALYSIS OF EXPRESSED PROTEINS BY METAPROTEOMICS WITH A FOCUS ON METHYLOBACTERIUM
C. Knief, N. Delmotte, G. Innerebner, J. Vorholt
ETH Zurich, Institute of Microbiology, Zurich, Switzerland

0237
A MARINE MICROBIAL COMMUNITY MAKES COMPLEX ORGANIC MATTER FROM A SIMPLE ORGANIC SUBSTRATE
E. Kujawinski, K. Barott, K. Longnecker
Woods Hole Oceanographic Institution, Marine Chemistry & Geochemistry, Woods Hole, United States of America

0238
AN ANALYSIS PIPELINE FOR THE PREDICTION OF FUNCTIONAL PROCESSES, TAXONOMIC STRUCTURE AND DIVERSITY OF MICROBIAL COMMUNITIES FROM THE METATRANSCRIPTOME
A. Lanzén¹, D.H. Huson², J. Qi³, S.C. Schuster³, C. Schleper⁴, T. Urich^4
1University of Bergen, Computational Biology Unit, BCCS, Bergen, Norway, ²Tübingen University, Center for Bioinformatics, Tübingen, Germany, ³Pennsylvania State University, Center for Comparative Genomics and Bioinformatics, University Park, United States of America, ⁴University of Vienna, Austria; University of Bergen, Center for Geobiology, Bergen, Norway

0239
THE EURYTHERMALISM OF AN EXTREME MICROBIAL SYMBIOSIS - AN ENZYMIC APPROACH
C.K. Lee¹, S.C. Cary², A.E. Murray³, R.M. Daniel^1
1University of Waikato, Thermophile Research Unit, Department of Biological Sciences, Hamilton, New Zealand, ²University of Delaware, College of Marine and Earth Studies, Lewes, United States of America, ³Desert Research Institute, Division of Earth and Ecosystem Sciences, Reno, United States of America

0240
ANALYSIS OF CHANGES IN GENE EXPRESSION IN SOIL EXPOSED TO 2,4-DICHLOROPHENOL USING RNA ARBITRARILY PRIMED PCR
L. Lillis, N. Clipson, E. Doyle
Microbial Ecology and Biodegradation Group, School of Biology and Environmental Science, University College Dublin, Dublin, Ireland

0241
PROBECHECK - A CENTRAL RESOURCES FOR EVALUATING OLIGONUCLEOTIDE PROBE COVERAGE AND SPECIFICITY
A. Loy¹, R. Arnold², P. Tischler², T. Rattei², M. Wagner¹, M. Horn^1
1Universität Wien, Department of Microbial Ecology, Wien, Austria, ²Technische Universität München, Department of Genome Oriented Bioinformatics, Freising, Germany

0242
SPECIES IDENTIFICATION OF DESCRIBED AND UNDESCRIBED MICROBIAL EUKARYTOES: CYTOCHROME C OXIDASE SUBUNIT 1 (COX-1) BARCODES AND CILIATED PROTISTS
D. Lynn, E. Gentekaki, C. Kher, M. Strueder-Kypke
University of Guelph, Department of Integrative Biology, Guelph, Canada

0243
RATIONALIZING PCR-PRIMER DESIGN FOR FUNCTIONAL ANALYSES - MAKING THE MOST OF PUBLIC DATABASES
J.L.S.G. Vianez Junior¹, I.C.N. Direito¹, N.S. Ferreira¹, A.G. O´Donnell², L.C.S. Mendonça - Hagler¹, A. Macrae^1
1UFRJ, Microbiologia Geral, Rio de Janeiro, Brazil, ²Newcastle University, IRES, Newcastle upon Tyne, United Kingdom

0244
QUANTIFICATION OF METHANOTROPHS IN RICE FIELDS BY STABLE ISOTOPE PROBING AND REAL-TIME PCR
D. Mayumi, N. Nomura, H. Uchiyama, T. Nakajima
Tsukuba University, Graduate School of Life and Environmental Sciences, Tsukuba, Japan

0245
QUANTIFICATION OF THE GENUS DEHALOCOCCOIDES USING ALTERNATELY BINDING PROBE COMPETITIVE PCR (ABC-PCR)
R. Miyata¹, K. Adachi², H. Tani², S. Kurata³, K. Nakamura¹, S. Tsuneda², Y. Sekiguchi¹, N. Noda^1
1National Institute of Advanced Industrial Science and Technology (AIST), Institute for Biological Resources and Functions, Tsukuba, Japan, ²Waseda University, Department of Chemical Engineering, Tokyo, Japan, ³J-Bio 21 Corporation, Tokyo, Japan

0246
SPECIFIC DETECTION OF PURPLE SULFUR BACTERIA USING PCR PRIMERS THAT TARGET DISSIMILATORY SULFITE REDUCTASE GENE
Y. Mori, R. Kondo
Fukui Prefectural University, Department of Marine Bioscience, Obama, Japan

0247
ISOLATION OF FUNCTIONAL GENES IN SOIL BY PCR-DGGE AND DNA WALKING
S. Morimoto, T. Fujii
National Institute for Agro-Environmental Sciences, Tsukuba, Japan

0248
EFFECTS OF LONG-TERM STEADY STATE CONDITIONS ON RESPIRATORY FLEXIBILITY OF PARACOCCUS DENITRIFICANS
A.P. Natale, K.J. Purdy
University of Warwick, Department of Biological Sciences, Coventry, United Kingdom

0249
A METAPROTEOMIC ANALYSIS OF MICROBIAL COMMUNITIES IN COASTAL WATERS
C. Ng¹, T. Thomas², F. Evans¹, M. Raftery³, R. Cavicchioli^1
1The University of New South Wales, School of Biotechnology and Biomolecular Sciences, Sydney, Australia, ²The University of New South Wales, Centre for Marine Bio-Innovation, Sydney, Australia, ³The University of New South Wales, Bioanalytical Mass Spectrometry Facility, Sydney, Australia

0250
APPLICATION OF A DNA MICROARRAY FOR MONITORING THE ORGANIC CONTAMINANT DEGRADATION POTENTIAL OF NATURAL MICROBIAL COMMUNITIES IN A CONSTRUCTED WETLAND
J. Ning¹, J. Liebich¹, J. Zhou², M. Kästner³, A. Schäffer⁴, P. Burauel^1
1Institute of Chemistry and Dynamics of the Geosphere (ICG) - Agrosphere, Research Center Jülich GmbH, Jülich, Germany, ²Institute for Environmental Genomics, University of Oklahoma, Norman, United States of America, ³Department of Bioremediation, Helmholtz Cetre for Environmental Research (UFZ), Leipzig, Germany, ⁴Institute for Environmental Research, RWTH Aachen University, Aachen, Germany

0251
PROFILING OF SOIL ARCHAEAL COMMUNITY BY REAL-TIME QUANTITATIVE PCR-BASED T-RFLP
T. Nishizawa¹, M. Komatsuzaki¹, N. Kaneko², H. Ohta^1
1Ibaraki University, College of Agriculture, Ami, Japan, ²Yokohama National University, Graduate School of Environment and Information Science, Yokohama, Japan

0252
COMPARATIVE ANALYSIS OF PROTEOMES EXPRESSED BY SINGLE-CELL OR FILAMENTOUS FORM OF SPHAEROLITUS NATANS
S. Lacroix¹, A. Guillot², T. Bouchez¹, C. Bureau¹, V. Monnet², J.-J. Pernelle^1
1Cemagref, HBAN, Antony, France, ²INRA, PAPSS, Jouy en Josas, France

0253
DISTRIBUTION AND DIVERSITY OF SOME MICROBIAL COMMUNITIES ASSESSED BY THE FUNCTIONAL GENES IN THE WATER COLUMN OF A MEROMICTIC LAKE (LAKE PAVIN, MASSIF CENTRAL, FRANCE)
C. Petit¹, G. Borrel², E. Peyretaillade², D. Jezequel³, E. Viollier⁴, P. Peyret², G. Fonty^5
1Microorganisms: Genome and Environment, UNM-CNRS 6023, Aubière, France, ²Microorganisms: Genome and Environment, UMR-CNRS 6023, Aubière, France, ³Laboratoire Géochimie des Eaux, UMR-CNRS, Paris, France, ⁴Laboratoire de Géochimie des Eaux, UMR-CNRS 7047, Paris, France, ⁵Microorganisms: Genome and Environment Laboratory, UMR-CNRS 6023, Aubière, France

0254
NANO SIP: STABLE ISOTOPE PROBING WITH NANOSIMS
J. Pett-Ridge
Lawrence Livermore National Lab, Chemical and Isotopic Signatures Group, Livermore, United States of America

0255
METABOLIC DESIGN: NEW BIOINFORMATIC TOOL FOR EFFICIENT DEVELOPMENT OF EXPLORATIVE FUNCTIONAL OLIGONUCLEOTIDE MICROARRAYS TO EVALUATE MICROBIAL METABOLIC PATHWAYS IN COMPLEX ENVIRONMENTS
S. Terrat¹, O. Bouzid¹, C. Petit¹, E. Dumas¹, E. Peyretaillade¹, F. Gravelat¹, O. Goncalves¹, G. Fonty¹, P. Pierre^2
1Microorganisms: Genome and Environment, UMR-CNRS 6023, Aubière, France, ²Microorganisms: Genome and Environment Laboratory, UMR-CNRS 6023, Aubière, France

0256
NEAR-FIELD PROBING OF VIBRATIONAL ABSORPTION IS AN EFFECTIVE TOOL FOR BIOMINERALIZATION ANALYSIS
M.A. Rahman, T. Oomori
University of the Ryukyus, Chemistry, Nishihara, Japan

0257
DIRECT SELECTION AND PHAGE DISPLAY OF BACTERIAL SECRETOME
D. Jankovic¹, M. Collett², M. Lubbers³, J. Rakonjac^1
1Massey University, Molecular BioSciences, Palmerston North, New Zealand, ²Fonterra Research Centre, Palmerston North, New Zealand, ³Fonterra, Mount Waverley, Australia

0259
ENVIRONMENTAL PROTEOMICS - IDENTIFICATION OF BIODEGRADATIVE ENZYMES AND CHARACTERIZATION OF MESOCOSM PROTEINS
T. Schneider¹, B. Gerrits², K. Riedel^1
1Institute of Plant Biology, University of Zurich, Microbiology, Zurich, Switzerland, ²Functional Genomics Center Zurich, Zurich, Switzerland

0260
COMPARING MICROBIAL GENE EXPRESSION PROFILES AMONG SPATIALLY SEPARATED MICROBIAL ASSEMBLAGES
Y. Shi¹, G. Tyson², J. Eppley², E. DeLong^2
1Massachusetts Institute of Technology, CEE, Cambridge, United States of America, ²Massachusetts Institute of Technology, Cambridge, United States of America

0261
IMPROVEMENT OF MEDIA FOR THERMOPHILIC AMMONIA-OXIDIZING BACTERIA IN COMPOST
C. Shimaya, T. Hashimoto
National Agricultural Research Center for Kyusyu Okinawa Region, Kumamoto, Japan

0262
HIGH TEMPERATURE-DIRECT VIABLE COUNT METHOD FOR ENUMERATION OF METABOLICALLY ACTIVE THERMOPHILIC BACTERIA IN COMPOST
K. Shiotsuka, K. Sakai
Kyushu University, Department of Plant Pesources, Graduate School of Bioresource and Bioenvironmental Sciences, Fukuoka, Japan

0263
LINKING STRUCTURE AND FUNCTION IN SYNTROPHIC BUTYRATE-DEGRADING CONSORTIA - INTEGRATING CULTIVATION AND RNA STABLE ISOTOPE PROBING
M. Altinbas¹, K. Roest², C.M. Plugge², I. Ozturk¹, A.J.M. Stams², H. Smidt^2
1Istanbul Technical University, Department of Environmental Engineering, Istanbul, Turkey, ²Wageningen University, Laboratory of Microbiology, Wageningen, Netherlands

0264
DENATURING GRADIENT GEL ELECTROPHORESIS ANALYSIS OF BACTERIAL COMMUNITY IN TOMATO RHIZOSPHERE TREATED WITH RHODOPSEUDOMONAS SP
H.-G. Song, K.-H. Lee
Kangwon National University, Biology, Chuncheon, Korea, Republic of

0265
ALTERNATELY BINDING PROBE COMPETITIVE POLYMERASE CHAIN REACTION FOR QUANTIFICATION OF THE GENE ENCODING AMMONIA MONOOXYGENASE
H. Tani¹, T. Kanagawa², S. Kurata³, T. Teramura¹, K. Nakamura⁴, S. Tsuneda¹, Y. Sekiguchi⁴, N. Noda^4
1Waseda University, Department of Life Science and Medical Bio-Science, Tokyo, Japan, ²Kyoto Gakuen University, Department of Bioenvironmental Design, Kyoto, Japan, ³J-Bio 21 Corporation, Tokyo, Japan, ⁴Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

0266
NEW APPLICATION OF THE ISOTOPE ARRAY USING RANDOM GENOMIC DNA PROBES DERIVED FROM A MICROBIAL COMMUNITY
T. Tobino¹, F. Kurisu², I. Kasuga¹, H. Furumai^2
1The University of Tokyo, Department of Urban Engineering, School of Engineering, Tokyo, Japan, ²The University of Tokyo, Research Center for Water Environment Technology, School of Engineering, Tokyo, Japan

0267
DIFFERENTIAL PROTEIN EXPRESSION DURING AEROBIC AND ANAEROBIC PHASES OF THE ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL PROCESS
M. Wexler¹, D. Richardson¹, P. Bond^2
1University of East Anglia, Norwich, United Kingdom, ²The University of Queensland, Brisbane, Australia

0268
ASSESSING THE TRANSCRIPTIONAL ACTIVITY OF SOIL MICROBIAL COMMUNITIES
P.M. Shrestha¹, M. Kube², R. Reinhardt², W. Liesack^1
1Max Planck Institute for Terrestrial Microbiology, Marburg, Germany, ²Max Planck Institute for Molecular Genetics, Berlin, Germany